The co-expression of human epidermal growth factor 2 and collagen Ⅳ in human breast cancer tissues detected by quantum dots-based immunofluorescence technique and its clinical significance
10.3760/cma.j.issn.1007-631X.2014.10.011
- VernacularTitle:纳米量子点标记探针技术对乳腺癌人表皮生长因子受体2及Ⅳ型胶原蛋白的原位标记成像及临床意义
- Author:
Jie MA
;
Shengrong SUN
;
Chuang CHEN
;
Jinzhong SUN
;
Yi TU
;
Min FANG
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Receptor,epidermal growth factor;
Collagen type Ⅳ;
Quantum dots
- From:
Chinese Journal of General Surgery
2014;29(10):775-778
- CountryChina
- Language:Chinese
-
Abstract:
Objective By using quantum dots-based double-color imaging method to simultaneously label human epidermal growth factor 2 (HER2) on breast cancer cell membrane and the type Ⅳ collagen in the extracellular matrix.To monitor the degree of malignancy of breast cancer and the invasive and metastatic potential.Methods The co-expressions of HER2 and the type Ⅳ collagen in breast cancer of 54 cases were detected and quantified by quantum dots-based double-labeling immunofluorescent histochemistry,and its correlation with clinical pathology parameters and prognosis were analyzed.Results With the increasing HER2 expression level,a progressive decrease in collagen Ⅳ around the cancer nest,the expression of HER2 and the type Ⅳ collagen value was negatively correlated (r =-0.980,P ＜ 0.05) ; the expression of HER2 and collagen Ⅳ was associated with lymph note metastasis,pathological stage (TNM) and disease-free survival(P ＜ 0.05).The expression of HER2 and the type Ⅳ collagen between different age groups,menopause and estrogen receptor (ER) and progesterone receptor (PR) were not significantly different (P ＞ 0.05).Conclusions Quantum dots-based double-color imaging method provides direct observable evidence to support the degradation of HER2 and the type Ⅳ collagen expression,which may help in determining the degree of malignancy and evaluating prognosis.
