Detection of Trichomonas vaginalis in male patients with urethritis by nested PCR
10.3760/cma.j.issn.0412-4030.2014.12.004
- VernacularTitle:巢式聚合酶链反应检测男性尿道炎患者尿液中阴道毛滴虫
- Author:
Wenjing LE
;
Xiaohong SU
;
Sai LI
;
Yurong LIU
;
Jinping ZHANG
;
Xiaofeng ZHU
;
Baoxi WANG
- Publication Type:Journal Article
- Keywords:
Urethritis;
Trichomonas vaginalis;
Polymerase chain reaction
- From:
Chinese Journal of Dermatology
2014;47(12):849-851
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish two nested PCR assays for detection of Trichomonas vaginalis in urine samples from male patients with urethritis,and to evaluate their diagnostic value.Methods One thousand and eighty-eight male patients with urethritis were enrolled from sexually transmitted disease (STD) clinic in the Hospital of Dermatology,Chinese Academy of Medical Sciences and Peking Union Medical College between April 2011 and December 2013.Urethral swabs were collected followed by smear testing,wet mount microscopic examination of Trichomonas vaginalis,and cultivation of Neisseria gonorrhoeae.Urine specimens were also obtained from these patients followed by DNA extraction.Two nested PCR assays were developed and performed to amplify the repeat genomic sequence and β-tubulin gene of Trichomonas vaginalis.Results Trichomonas vaginalis was detected in none of these swab specimens by wet mount microscopy,but in 29 (2.67％) of the urine specimens by either of the two nested PCR assays.Moreover,the positive specimens detected by the two nested PCR assays were completely consistent.Conclusion Compared with wet mount microscopy,nested PCR has higher sensitivity and specificity in detection of Trichomonas vaginalis in urine samples from male patients.
