Effect of Notch1 gene knockdown on migration and proliferation of human prostate cancer cell line PC-3
10.3760/cma.j.issn.1006-9801.2014.11.001
- VernacularTitle:下调Notch1基因对前列腺癌PC-3细胞迁移和增殖的影响
- Author:
Jin SIMA
;
Bao ZHANG
;
Baojun WANG
;
Qing AI
;
Xin MA
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
Prostate neoplasms;
Notch1 gene;
Hes1 gene;
Cell migration;
Cell proliferation
- From:
Cancer Research and Clinic
2014;26(11):721-724
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Notch1 gene dowuregulated on migration and proliferation of human prostate cancer cell line PC-3.Methods The small interfering RNA (siRNA) targeted Notch1 gene or negative control sequences was transfected into PC-3 cells.The expression of Notch1 or Hes1 gene was detected by Real-Time PCR and Western Blot.Then ability of migration or proliferation was measured by Transwell assay or MTS Assay.Results Compared with negative control group (36.097±1.941) and untransfected group (38.762±1.897),Notch1 expression level in siRNA group (3.960±0.510) was significantly reduced (P ＜ 0.01).Meanwhile,Hes1 level in siRNA group was decreased,expression in three groups as follows:siRNA group was 1.690±0.994,negative control group was 8.776±0.916,untransfected group was 9.803±1.001 (P ＜ 0.01).In Transwell assay,the number of migration cells in siRNA group was 657.867±27.610,more than that in the negative control group (158.533±18.263) and untransfected group (146.933±15.733) (P ＜ 0.01).In MTS assay,there was no significant difference among three groups at 0 h point,however,siRNA group was significantly raised at the time points of 24,48 and 72 h (P ＜ 0.01).Conclusions Downregulation of Notch1 gene by transfection of the siRNA-Notch1 sequences significantly promoted ability of migration or proliferation in PC-3 cells,and the effect may be due to the down-regulation of Hes1 expression.
