Effect of miR-218 on biological functions of renal cell carcinoma cell line
10.3760/cma.j.issn.1006-9801.2014.10.001
- VernacularTitle:miR-218对肾癌细胞生物学功能的影响
- Author:
Long HE
;
Hongwei YANG
;
Yan ZHANG
;
Jun WANG
;
Long LIU
;
Lianhui FAN
- Publication Type:Journal Article
- Keywords:
Carcinoma,renal cell;
miR-218;
Apoptosis;
Proliferation;
Anti-oncogene
- From:
Cancer Research and Clinic
2014;26(10):649-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of miR-218 on biological functions of renal cell carcinoma cell line through regulating the expression levels of miR-218.Methods The pcDNA3.1-miR-218 was transfected into renal carcinoma cell line A498 and 769-P and the expression of miR-218 was detected.The cell activity,invasion,apoptosis and proliferation of transfected renal carcinoma cell line were analyzed in vitro.Results After plasmid transfected A498/769-P renal carcinoma cell line,the expression level of miR-218 (1.99,1.64) was significantly higher than that of the control group (1.00) (t =60.82,10.89,P ＜ 0.000 1) The cell viability (0.90±0.10,0.68±0.06) was lower than that of the control group (1.39±0.14,1.24±0.08) by CCK8 experiment (t =15.02,31.69,P ＜ 0.000 1).The cell invasion was lower than that of control group by Transwell experiment (t =15.78,18.80,P ＜ 0.000 1).The apoptosis ratio was higher by AnnxinV-FITC experiment,the apoptosis ration of control group and transfected group were respectively 0.25 ％,45.77 ％ in A498 cell line and 0.11％,45.57 ％ in 769-P cell line.The proliferation was lower than that of the control group (P ＜ 0.000 1).Conclusion Up-regulation of miR-218 expression can inhibit the growth of renal cell carcinoma cell line in vitro.
