Role of β-catenin signaling pathway in the epithelial to mesenchymal transition induced by parathyroid hormone in renal tubular epithelial cells
10.3760/cma.j.issn.1001-7097.2014.10.007
- VernacularTitle:β连环素信号通路介导甲状旁腺素诱导的人近曲小管上皮细胞转分化
- Author:
Yunshan GUO
;
Aiping ZHANG
;
Yanxia WANG
;
Lei ZHANG
;
Hongdong LI
;
Zhen LI
;
Yaohai DING
- Publication Type:Journal Article
- Keywords:
Beta Catenin;
Fibrosis;
Parathyroid hormone;
Epithelial to mesenchymal transition;
Renal tubular epithelial cells
- From:
Chinese Journal of Nephrology
2014;30(10):763-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of parathyroid hormone (PTH) on the epithelial to mesenchymal transition (EMT) in human renal proximal tubular epithelial cells (HK-2 cells),and determine the role of β-catenin signaling pathway.Method The expression of α-smooth muscle actin (α-SMA),E-cadherin and β-catenin in HK-2 cells was measured by real-time PCR,Western blotting and immunofluorescence technique.The signaling pathway by which PTH activated EMT in HK -2 cells was identified by using synthetic β-catenin siRNA.Results Parathyroid hormone (10-10mol/ L) increased α-SMA expression and decreased E-cadherin expression in HK-2 cells (P＜ 0.01,respectively).Untreated cells showed the expression of E-cadherin,whereas α-SMA staining was noticeably increased in cells treated with PTH.β-catenin activity was significantly increased after exposed to PTH.Theα-SMA expression was decreased strongly and E-cadherin expression was increased after β-catenin siRNA transfection (all P ＜ 0.05).Conclusion PTH significantly induces epithelial to mesenchymal transition in HK-2 cells throughβ-catenin signaling pathway.
