Effect of glyburide on the proliferation, differentiation, and apoptosis of MC3T3-E1 osteoblasts under high glucose

VernacularTitle:格列苯脲在高糖环境下对MC3T3-E1成骨细胞增殖、分化和凋亡的影响
Author: Juan ZHANG ; Huijuan ZHANG ; Shuang JIN ; Wei ZHANG ; Haofeng ZHOU
Publication Type:Journal Article
Keywords: Glyburide; MC3T3-E1 osteoblasts; Proliferation; Differentiation; Apoptosis
From: Chinese Journal of Endocrinology and Metabolism 2015;31(5):462-464
CountryChina
Language:Chinese
Abstract: After MC3T3-E1 cells were treated with 1,10,and 20 μmol/L glibenclamide for 48 h,the proliferation rate of cells was detected by CCK-8 assay.Flow cytometry was used to test cell apoptosis.The mRNA expressions of collagen I (COL-1) and osteopontin (OPN) were tested by realtime fluorescence quantitative PCR.Western blot was used to detect the expression levels of apoptosis-related proteins Bax and Bcl-2.The results showed that compared with the control group,the proliferation rate of MC3T3-E1 cells was gradually increased (P＜0.05),the apoptosis rate decreased (P ＜ 0.05),the expressions of COL-1 mRNA,OPN mRNA,and Bcl-2 protein were progressively raised (P＜0.05),and the expression of Bax protein were gradually decreased (P＜0.05) along with increasing concentration of glyburide.It suggested that glibenclamide could promote the proliferation and differentiation of MC3T3-E1 cells in high glucose and may inhibit apoptosis in a concentration-dependent manner within a certain range.