Effects of forkhead transcription factor O1 on the expression of type Ⅳ collagen and desmin in podocytes of diabetic rats
10.3760/cma.j.issn.1000-6699.2015.04.015
- VernacularTitle:叉头状转录因子O1对糖尿病大鼠足细胞Ⅳ型胶原和desmin表达的影响
- Author:
Yuanyuan ZHANG
;
Qingzhu WANG
;
Guijun QIN
;
Yingni ZHOU
;
Xiaojun MA
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Forkhead transcription factor O1;
Podocytes;
Type Ⅳ collagen;
Desmin
- From:
Chinese Journal of Endocrinology and Metabolism
2015;31(4):354-359
- CountryChina
- Language:Chinese
-
Abstract:
To study the effects of forkhead transcription factor O1 (FoxO1) on the expression of type Ⅳ collagen and desmin in podocytes of diabetic rats.Streptozotocin-induced diabetic rats were divided into three groups:diabetic rats (DM group),rats transfected with blank lentiviral vectors (diabetes mellitus plus LV-pSC-GFP group,LV-NC group),and rats which were transfected with lentiviral vectors carrying constitutively active FoxO1 (diabetes mellitus plus LV-FoxO1-AAA group,LV-CA group).Rats which received an injection of diluent buffer served as normal control.At 2,4,and 8 weeks after transfection,the levels of urine albumin,blood glucose,blood urea nitrogen,and serum creatinine were measured.Realtime PCR and Western blotting were performed to measure the mRNA and protein levels of FoxO1,COL4A3,COL4A5,and desmin in the renal cortex.Moreover,light microscope and electron microscope were used to observe the structural changes in glomerulus and podocytes.Compared with LV-NC and DM group,in LV-CA group,there was a significant increase in the mRNA and protein levels of FoxO1,and a distinct decrease in the levels of urine albumin,blood urea nitrogen and serum creatinine of rats (except at the twoweek time point) (all P＜0.05),the mRNA and protein levels of COL4A3,COL4A5,and desmin were all decreased (all P＜0.05),and pathological changes in kidney were also improved.Upregulating the expression of FoxO1 by transfecting with constructed lentiviral vectors can definitely improve the abnormal expression of type Ⅳ collagen and desmin in podocytes of diabetic rats.
