The intervention of glucagon-like peptide 1 analogue in IRE1α-JNK signaling pathway of rats with non-alcoholic fatty liver disease
10.3760/cma.j.issn.1000-6699.2015.03.018
- VernacularTitle:胰升糖素样肽1类似物对非酒精性脂肪肝大鼠IRE1α-JNK通路的干预效应
- Author:
Jiayu XU
;
Na AO
;
Jian DU
;
Jing YANG
;
Xiaochen WANG
- Publication Type:Journal Article
- Keywords:
Glucagon-like peptide 1;
Non-alcoholic fatty liver disease;
Endoplasmic reticulum stress;
Rats;
Inositol requiring enzyme-1α;
c-jun N-terminal kinase
- From:
Chinese Journal of Endocrinology and Metabolism
2015;31(3):272-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expressions of endoplasmic reticulum stress (ERs) related factors including inositol requiring enzyme-1α(IRE1αα),p-IRE1 α,c-jun N-terminal Kinase(JNK),and p-JNK in rats with non-alcoholic fatty liver disease,and to investigate the effect of intervention with glucagon-like peptide 1 (GLP-1) analogue.Methods Forty male Sprague-Dawley rats were divided into normal chow group(n =15) and high-fat diet group(n=25).After 12 weeks,5 rats of each group were used to assess the establishment of rat models with non-alcoholic fatty liver disease.Then the high-fat diet group rats were divided into high-fat diet group (HF,n =10) and GLP-1 group(HG,n=10) and treated with normal saline and GLP-1 analogue for4 weeks respectively.Body weight and biochemical markers in rats were measured.The expressions of IRE1α,p-IRE1α,JNK,and p-JNK were measured by Western blot.Results Compared with the NC group,the levels of body weight,plasma triglyceride (TG),total cholesterol (TC),low density lipoprotein-cholesterol (LDL-C),alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in HF group were significantly higher (all P ＜ 0.01),high density lipoprotein-cholesterol(HDL-C) was decreased(P＜0.01),and p-IRE1 α/IRE1 α and p-JNK/JNK were increased(P＜0.05 and P＜0.01).After GLP-1 treatment,body weight,plasma TG,TC,LDL-C,AST,ALT in HF group were significantly lowered(P＜0.05 or P＜0.01),HDL-C was increased(P＜0.01),p-IRE1 α/IRE1 α and p-JNK/JNK were decreased (P＜0.05 and P＜0.01).Conclusion GLP-1 analogue may improve hepatic steatosis via regulating ERs related IRE1 α-JNK signaling pathway.
