Efficacy of Polymerase Chain Reaction in Early Diagnosis of Herpes Simplex Keratitis in Rabbit Model.
- Author:
Jae Yong HEO
1
;
Su Jin KIM
;
Eun Mi SHIN
;
Woo Jin SAH
;
Tae Won HAHN
Author Information
1. Department of Ophthalmology, College of Medicine, The Catholic University of Korea, Korea.
- Publication Type:Original Article
- Keywords:
Herpes simplex keratitis(HSK);
Polymerase chain reaction(PCR);
Rabbit model
- MeSH:
Cornea;
Early Diagnosis*;
Herpes Simplex*;
Keratitis, Herpetic*;
Polymerase Chain Reaction*;
Rabbits;
Sensitivity and Specificity
- From:Journal of the Korean Ophthalmological Society
2001;42(1):137-144
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We evaluated the efficacy of polymerase chain reaction(PCR)in early diagnosis of herpes simplex keratitis(HSK)in rabbit model and compared the sensitivity and specificity of PCR in two kinds of primers. Only one eye of 8 rabbits was inoculated with HSV Type 1(F strain, ATCC VR-73)to induce HSK, and the other eye was used as control. Rabbit cornea was examined under slit lamp examination and PCR test of tear and corneal scraping specimens were performed at 1, 3, 5, 7, and 10th post-inoculation day. The sensitivity of PCR with POL primer was 100%in tear and corneal scraping specimens. The sensitivity of PCR with POL primer and LAT primer were 80%and 100%respectively. PCR test is very useful diagnotic tool for the early diagnosis of HSK in rabbit model. In addition, PCR test with corneal scraping specimens provided better yield compared with tears.
