Antioxidant Activity of Xanthium Strumarium L. Extracted F ractions in Lens Epithelial Cells.
- Author:
Kwang Won LEE
1
;
Seok Jong LEE
;
Hyun Kyung PARK
;
Myung Jin KIM
;
Young Shin CHUNG
;
Eun Kyung HONG
;
Choun Ki JOO
Author Information
1. Department of Ophthalmology, Kangnam St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Korea.
- Publication Type:Original Article
- Keywords:
Cataract;
Lens epithelial cells;
Antioxidant;
Oxidative stress
- MeSH:
Antioxidants;
Cataract;
Cell Culture Techniques;
Cell Survival;
Chrysanthemum;
Epithelial Cells*;
Fruit;
Humans;
Oxidative Stress;
Solvents;
Xanthium*
- From:Journal of the Korean Ophthalmological Society
2001;42(1):152-159
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To elucidate the antioxidant effect of Xanthium strumarium L., a fruit of Dokomari or Daekori, which is a family of chrysanthemum on the H2O2-mediated cellualr damage, we examined the effect of Xanthium strumarium L. extraction fractions on survival of human lens epithelia, HLE B-3 cells by using cell culture system. H2O2-mediated cellualr death and its IC5 0, with approximately 100 micrometer H2O2 were determined by using MTT assay. The HLE B-3 cells pretreated with Xanthium strumarium L. extract fractions, were incubated with 100 micrometer H2O2, and in order to assess the cell viability the cultures were incubated with MTT solution. Among Xanthium strumarium L. extract fractions, Xan crude fraction, Xan CHCl3 fraction, and Xan EtAc fraction have antioxidant activity at the concentrations of 500 ng/ml, 1 microgram/ml and 100 ng/ml, respectively. These effects were statistically significant(p<0.05). Also, the fact that only the frations extracted with organic solvents have antioxidant activity suggests that the active components from the extract have hydrophobic property.
