Effect of berberine preconditioning on intestinal injury caused by liver cold ischemia/reperfusion in rats
10.3760/cma.j.issn.0254-1416.2014.11.007
- VernacularTitle:小檗碱预处理对大鼠肝脏冷缺血再灌注时肠损伤的影响
- Author:
Wenna LIU
;
Hongyin DU
;
Wenli YU
;
Yiqi WENG
;
Yongwang WANG
;
Yuliang WANG
- Publication Type:Journal Article
- Keywords:
Berberine;
Reperfusion injury;
Liver;
Intestines
- From:
Chinese Journal of Anesthesiology
2014;34(11):1312-1315
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of berberine preconditioning on the intestinal injury caused by liver cold ischemia/reperfusion (I/R) in rats.Methods Twenty-four pathogen-free male Sprague-Dawley rats,weighing 220-250 g,were randomized into 3 groups (n =8 each) using a random number table:sham operation group (S group),I/R group,and berberine preconditioning group (B group).The animals were anesthetized with 5％ chloral hydrate 60 mg/kg.In B group,berberine 200 mg/kg was administered through a gastric tube once a day for 7 consecutive days starting from 7 days before operation.The equal volume of normal saline was given instead of berberine in group Ⅰ/R.At 8 h after operation,the rats were sacrificed and the intestines were harvested for determination of wet/dry lung weight ratio (W/D ratio),levels of malondialdehyde (MDA) and superoxide dismutase (SOD),expression of cytochrome C,Bax and Bcl-2 and apoptotic cell count,and for microscopic examination.Intestinal damage was assessed and scored according to Chiu.Results Compared with group S,Chiu's score,W/D ratio,MDA content and apoptotic cell count were significantly increased,SOD activity was decreased,the expression of cytochrome C and Bax was up-regulated,and the expression of Bcl-2 was downregulated in I/R and B groups.Compared with group I/R,Chiu's score,W/D ratio,MDA content and apoptotic cell count were significantly decreased,SOD activity was increased,the expression of cytochrome C and Bax was down-regulated,and the expression of Bcl-2 was up-regulated in B group.Conclusion Berberine preconditioning can reduce the intestinal injury caused by liver cold I/R in rats,and the mechanism is related to inhibition of lipid peroxidation and cell apoptosis.
