Effects of serum in patients with obstructive jaundice on proliferation, migration and phenotype modulation of human pulmonary arterial smooth muscle cells
10.3760/cma.j.issn.0254-1416.2014.10.031
- VernacularTitle:梗阻性黄疸患者血清对人PASMCs增殖、迁移及表型转换的影响
- Author:
Yong YANG
;
Bing CHEN
;
Yang CHEN
;
Baoli ZU
;
Bin YI
;
Kaizhi LU
- Publication Type:Journal Article
- Keywords:
Jaundice,obstructive;
Myocytes,smooth muscle;
Lung;
Cell proliferation;
Cell movement;
Cell differentiation
- From:
Chinese Journal of Anesthesiology
2014;34(10):1271-1274
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of the serum in the patients with obstructive jaundice on the proliferation,migration and phenotype modulation of human pulmonary arterial smooth muscle cells (PASMCs).Methods PASMCs were isolated from the patients,underwent passage and were then subcultured.The cultured PASMCs were incubated with the serum in the patients with obstructive jaundice or with the serum in the healthy volunteers.At 24,48 and 72 h of incubation,the proliferation and migration of the cells were determined by CCK-8 assay and wound healing assay,respectively,and Western blot analysis was used for detection of smooth muscleα-actin (SM-α-actin) and calponin protein expression in human PASMCs.Results The proliferation and migration of human PASMCs were significantly enhanced,calponin protein expression was up-regulated at 24 h of incubation,and the SM-α-actin and calponin protein expression was down-regulated at 48 and 72 h of incubation when human PASMCs were incubated with the serum in the patients with obstructive jaundice as compared with those when the cells were incubated with the serum in the healthy volunteers.When human PASMCs were incubated with the serum in the patients with obstructive jaundice,the proliferation and migration of the cells were significantly enhanced,SM-α-actin expression was up-regulated and calponin protein expression was down-regulated at 48 h of incubation as compared with those at 24 h of incubation.When human PASMCs were incubated with the serum in the patients with obstructive jaundice,the migration of the cells was significantly enhanced,and no significant change was found in the proliferation and SM-α-actin and calponin protein expression at 72 h of incubation as compared with those at 48 h of incubation.Conclusion The serum in the patients with obstructive jaundice can enhance the proliferation and migration of human PASMCs and promotes synthetic PASMC phenotype.
