Expressions of Beclin 1 and its interacting proteins in 3T3-L1 differentiation and subcutaneous/visceral fat
10.3760/cma.j.issn.1000-6699.2014.12.015
- VernacularTitle:Beclin 1及其相互作用蛋白在脂肪细胞分化及内脏和皮下脂肪组织中的表达
- Author:
Jian YANG
;
Pengfei LYU
;
Xiaoyan ZHANG
;
Yujie DENG
;
Ying YANG
- Publication Type:Journal Article
- Keywords:
Autophagy;
Beclin 1;
Adipose;
Differentiation
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(12):1108-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expressions of Beclin 1 and its 11 interacting proteins in subcutaneous fat and visceral fat,and to observe the expressions of these genes in 3T3-L1 adipocytes differentiation process.Methods In 3T3-L1 adipocytes,cells were harvested at day 0,2,4,6,8 in differentiation process,and then proteins or mRNAs were obtained and followed with Western blot and realtime PCR.Mice tissue proteins or mRNAs were detected with Western blot and realtime PCR.Results In 3T3-L1 adipocytes differentiation process,expressions of LC3,Beclin 1,and TNFAIP3 continued to rise,expression of BIRC5 was high to low,FEZ1 continued at a low level.In mice,expressions of LC3,Beclin 1,YWHAQ,FEZI,BAD,WAC,TNFAIP3,and GOPC in visceral fat were significantly higher than those in subcutaneous fat,while expression of SLAMF1was higher in subcutaneous fat.Conclusion Autophagy plays an important role in 3T3-L1 differentiation process and may be tissue-specific in visceral fat and subcutaneous fat.
