Maternal subclinical hypothyroidism during pregnancy down-regulates insulin-like growth factor Ⅰ signaling pathway on hippocampus in rat offspring
10.3760/cma.j.issn.1000-6699.2014.12.008
- VernacularTitle:大鼠妊娠期母体亚临床甲状腺功能减退下调后代海马胰岛素样生长因子Ⅰ通路相关蛋白水平
- Author:
Yuxin FAN
;
Yuanyuan ZHANG
;
Suqing BAO
;
Chenling FAN
;
Weiping TENG
;
Zhongyan SHAN
- Publication Type:Journal Article
- Keywords:
Pregnancy;
Subclinical hypothyroidism;
Insulin-like growth factor Ⅰ;
Insulin-like growth factor Ⅰ receptor;
Phosphorylated-protein kinase B;
Morris water maze;
Long-term potentiation
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(12):1068-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of subclinical hypothyroidism during pregnancy on hippocampus insulin-like growth factor Ⅰ (IGF-Ⅰ) signaling pathway in rat offspring.Methods A total of 60 female Wistar rats were evenly divided into control(CON),subclinical hypothyroidism(SCH),and clinical hypothyroidism (CH) groups.The hippocampus of progenies were collected on the postnatal day 3,postnatal day 7 to measure protein kinase B (Akt) and phosphorylated-Akt (p-Akt) by Western blot,IGF-Ⅰ and insulin-like growth factor Ⅰ receptor (IGF-Ⅰ R) by Elisa.Morris water maze and field excitatory postsynaptic potential long-term potentiation were measured at the postnatal 40 day.Results Western blot and Elisa revealed that levels of IGF-Ⅰ,IGF-Ⅰ R,and p-Akt of pups from SCH group were lower than that of CON group and were higher than CH group on day 3 (P ＜ 0.05).On day 7,the levels of IGF-Ⅰ,IGF Ⅰ R,and p-Akt of pups from SCH group were lower than CON group (P＜ 0.05),but no difference was observed in p-Akt and IGF-Ⅰ R level between SCH group and CH group (P ＞ 0.05).Latencies of all groups had shortened in Morris water maze test with increasing of training trials.The slope of field excitatory postsynaptic potenial was increased in all groups after Theta burst stimulation.The amplification percentage of slope of field excitatory postsynaptic potenial in SCH group's was lower than control group's but was higher than CH group's(all P values＜0.05).Conclusions Maternal subclinical hypothyroidism impairs long-term potentiation induction in hippocampus of rat might be associated with the levels of IGF-Ⅰ,IGF-Ⅰ R,and p-Akt.
