Resveratrol improves intestinal injury in hemorrhagic shock rats by protection of mitochondria and reduction of oxidative stress
10.11817/j.issn.1672-7347.2014.12.007
- VernacularTitle:白藜芦醇通过保护线粒体和减少氧化应激改善失血性休克大鼠肠损伤
- Author:
Shaofan KE
;
Feng LIU
;
Zhuanghui ZHU
- Publication Type:Journal Article
- Keywords:
resveratrol;
hemorrhagic shock;
intestinal injury;
oxidative stress;
mitochondria
- From:
Journal of Central South University(Medical Sciences)
2014;(12):1259-1265
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore whether resveratrol can reduce intestinal damage in hemorrhagic shock rats and the underlying mechanism. Methods: A total of 24 Sprague-Dawley rats of speciif c pathogen free (SPF) were randomly divided into a control group(n=8), a resveratrol group (SR group,n=8) and a vehicle group (SS group,n=8). hT e mean arterial pressure was recorded. Two hours atf er hemorrhagic shock, 15 mg/kg resveratrol or 0.3 mL equal volume of vehicle and autologous blood were given, respectively. The intestinalspecimens were collected for hematoxylin-eosin (HE) staining and calculated the pathological score. The superoxide dismutase 2 (SOD2) and cytochrome C (Cyt C) protein expression was examined by immunohistochemistry and/or Western blot. ATP level, activities of glutathione peroxidase (GXH-px), catalase (CAT) and SOD were also detected. Results: Two hours atfer autologous blood transfusion, the mean arterial pressure in the SR group was signiifcantly higher than that in the SS group (P<0.01). Compared with the SS group, the pathological injury was signiifcantly alleviated and pathological scores were dramatically reduced in the SR group (P<0.05). hTe activities of GXH-px, CAT, SOD and the ATP levels in the SR group were signiifcantly higher than those in the SS group (allP<0.01). Compared with the SS group, the SOD2 expression was significantly higher while the Cyt C expression was dramatically lower in the SR group (both P<0.01). Conclusion: Resveratrol could alleviate the intestinal injury in hemorrhagic shock rats, which might be associated with its effects on reduction of oxidative stress and protection of mitochondria.
