Effects of tumor necrosis factor-α converting enzyme on mucous hypersecretion in inflammatory airway
10.11817/j.issn.1672-7347.2014.12.002
- VernacularTitle:肿瘤坏死因子转换酶在炎性气道黏液高分泌中的作用
- Author:
Xuelin LI
;
Haiqiao WU
;
Xia CHEN
- Publication Type:Journal Article
- Keywords:
TNF-α converting enzyme;
MUC5AC;
human neutrophil elastae;
TNF-α converting enzyme inhibitor-1
- From:
Journal of Central South University(Medical Sciences)
2014;(12):1228-1232
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of tumor necrosis factor-α converting enzyme (TACE) on mucous hypersecretion in inlf ammatory airway. Methods: Mucous hypersecretion model of human lung adenocarcinoma cells A549 was induced by human neutrophil elastase (HNE), and TNF-α converting enzyme inhibitor-1 (TAPI-1), an inhibitor of TACE, was chosen for the inference study. The expression of MUC5AC and TACE was examined. hT e cells were divided into 5 groups: a negative control group, HNE1 (15 nmol/L) group, HNE2 (25 nmol/L) group, HNE3 (50 nmol/L) group and TAPI-1 group. RT-PCR was used to examine MUC5AC and TACE mRNA expression. The protein expression of TACE and MUC5AC was examined by Western blot and ELISA, respectively. Results: HNE induced the TACE and MUC5AC mRNA and protein expression in a dose-dependent manner. Compared with the control group, the increases were all signiifcantly increased in the three dosages of HNE group (P<0.01). The HNE-induced TACE and MUC5AC mRNA and protein expression were dramatically attenuated in the presence of TAPI-1, an inhibitor of TACE (P<0.01). Conclusion: TACE participated cell signalling pathway of airway mucous hypersecretion, and could down regulation the level of inlfammation airway mucous hypersecretion.
