Puerarin promotes the proliferation of osteoblasts in older female patients with osteoporosis
10.3969/j.issn.2095-4344.2015.29.001
- VernacularTitle:葛根素可促进老年女性骨质疏松症患者成骨细胞的增殖
- Author:
Yumin SUN
;
Xiaolin XU
;
Yi YANG
;
Dongdong HUANG
;
Jinxiu XU
;
Cai CHENG
;
Lu WANG
- Publication Type:Journal Article
- Keywords:
Puerarin;
Osteoporosis;
Osteoblasts;
Proliferation
- From:
Chinese Journal of Tissue Engineering Research
2015;(29):4593-4597
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In recent years, there are many animal studies and osteoblast studies on the anti-osteoporotic effects of puerarin, a kind of phytoestrogen. But few of them are reported on the effects of puerarin on osteoblasts in older patients with osteoporosis. OBJECTIVE: To observe the influence of puerarin on the proliferation ofin vitro cultured osteoblasts from older female patients with osteoporosis. METHODS:The older female patients with osteoporotic femoral neck fractures who underwent artificial femoral head replacement were included in this study. The femoral neck cancelous bone removed during the operation was colected. Primary cancelous bone osteoblasts were cultured using explant culture method. The cels were sub-cultured to the required amounts. Osteoblasts from the control group were cultured with culture medium without puerarin. Osteoblasts from the 0.01, 0.1, and 1 μmol/L puerarin groups were cultured with culture medium containing the corresponding concentrations of puerarin. After in vitro co-culture with different concentrations of puerarin for 1, 3, 5 days, the proliferation of osteoblasts was observed. RESULTS AND CONCLUSION:With the increase in the concentration of puerarin, the proliferative activity of
osteoblasts constantly increased at different time points (P < 0.05). At 3 days of culture, cel absorbance value in each group reached the peak level. These results suggest that 0.01, 0.1, 1μmol/L puerarin promotes the proliferation of osteoblasts in older patients with osteoporosis in a concentration-dependent manner.
