Establishing an animal model of delayed onset muscle soreness and its histomorphologic observation
10.3969/j.issn.2095-4344.2015.29.011
- VernacularTitle:延迟性肌肉酸痛动物模型的建立及组织形态学观察
- Author:
Yuan WEI
;
Chunlu FANG
;
Liangming LI
;
Wenhua XING
;
Zeyi YANG
- Publication Type:Journal Article
- Keywords:
Sports;
Muscle,Skeletal;
Desmin;
Vimentin
- From:
Chinese Journal of Tissue Engineering Research
2015;(29):4645-4651
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Delayed onset muscle soreness is closely related to skeletal muscle micro-injury, but the exact mechanism of skeletal muscle micro-injury is not yet clear. OBJECTIVE:To observe the histomorphological and ultrastructure changes of skeletal muscle micro-injury models induced by eccentric exercise. METHODS: Forty male Sprague-Dawley rats were randomly divided into quiet control group, immediately after exercise group, post-exercise 24 hours group, post-exercise 48 hours group and post-exercise 72 hours group. In the latter four groups, the rats were subjected to intermittent running on the-16° slope at a speed of 16 m/min: 5 minutes movement, 2 minutes rest and totaly 120 minutes. Rats in the latter four groups were observed immediately, at 24, 48, 72 hours after exercise. RESULTS AND CONCLUSION:After eccentric exercise, the morphology and ultrastructure of rat’s skeletal muscle were both changed to different extents, and Desmin and Vimentin were dyed off for anti-desmin antibody staining at varying degrees. It indicates that one-time eccentric exercise can cause delayed skeletal muscle micro-injury.
