Effect of Rab11 on biological functions of cervical cancer cell line HeLa
10.3760/cma.j.issn.1006-9801.2015.07.002
- VernacularTitle:Rab11对子宫颈癌HeLa细胞生物学功能的影响
- Author:
Jianhua ZHANG
;
Longzhen ZHANG
;
Yanyan KAN
;
Chunhua YANG
;
Min ZHOU
;
Jingjing ZHAO
;
Xia WANG
- Publication Type:Journal Article
- Keywords:
Cervical neoplasms;
Rab11;
Proliferation;
Invasion;
HeLa cell line
- From:
Cancer Research and Clinic
2015;(7):438-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Rab11 on biological functions of human cervical cancer cell line HeLa through regulating the expression levels of Rab11. Methods The Rab11 siRNA was transfected into HeLa cells and the expression of Rab11 was detected by Western blot. CCK8 assay, colony formation experiments, EdU assay and Transwell assay were adopted to observe the effect of Rab11 on HeLa cells proliferation and invasion. Results The expression of Rab11 was decreased significantly in HeLa cells transfected with Rab11 siRNAs than that in control siRNA (1.096 ±0.091 vs 1.735 ±0.084, P< 0.01). The proliferation was markedly inhibited in Rab11 siRNA group compared with that in control siRNA group (48 h:0.721±0.092 vs 1.090±0.099; 72 h: 0.956±0.105 vs 1.482±0.096; 96 h: 1.231±0.099 vs 1.720±0.174, P< 0.01), the number of colonies was lower than that in control siRNA group (36±1 vs 75±8, P< 0.01) and so was proliferation rate [(33.880±1.902) % vs (45.570±2.025) %, P< 0.05]. The cell invasion rate of Rab11 siRNA group was lower than that of control siRNA group [(38.6 ±0.8) % vs (100.0 ±0.2) %, P< 0.01]. Conclusion Down-regulation of Rab11 expression can inhibit the growth of HeLa cells.
