Suppression of hepatocellular carcinoma invasion and metastasis by Rho-kinase inhibitor Fasudil through inhibition of BTBD7-ROCK2 signaling pathway
10.11817/j.issn.1672-7347.2014.12.001
- VernacularTitle:Rho激酶抑制剂法舒地尔作用BTBD7-ROCK2信号通路抑制肝细胞癌侵袭运动
- Author:
Kuan HU
;
Zhiming WANG
;
Yiming TAO
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
BTB/POZ domain containing 7;
Rho-associated,coiled-coil containing protein kinase 2;
Fasudil;
invasion and metastasis
- From:
Journal of Central South University(Medical Sciences)
2014;(12):1221-1227
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the eff ect of Fasudil on the invasion and metastatic abilities of human high metastatic liver cancer cells (HCCLM3) and the underlying mechanisms. Methods: HCCLM3 cells were incubated with 100 μmol/L Fasudil. Fluorescence staining forF-actin and Transwell assay were performed to observe the invasion ability of HCCLM3 cells. HCCLM3 cells were divided into 3 groups: a negative control group, a Fasudil group and a BTB/POZ domain containing 7 (BTBD7)-siRNA group. Western blot assay was performed to detect the expression levels of BTBD7, ras homolog family member C (RhoC) and Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), matrix metalloproteinases 2 (MMP2) and MMP9. Zymogram analysis method was performed to detect the expression activities of MMP2 and MMP9. hTe BTBD7-siRNA group was served as a positive control. Results: In HCCLM3 cells treated with Fasudil, the invasion ability was significant decreased compared with the control group, concomitant with the down-regulated expression levels of BTBD7, RhoC and ROCK2 protein as well as the decreased activities of MMP2 and MMP9. Conclusion: Fasudil plays an important role in interfering BTBD7-ROCK2 signaling pathway and suppressing the invasion and metastasis of hepatocellular carcinoma.
