Research on inflammatory response of Helicobacter pylori-treated macrophages
10.3969/j.issn.1000-484X.2015.06.004
- VernacularTitle:幽门螺杆菌作用巨噬细胞后诱导的炎症反应研究
- Author:
Jianjun WANG
;
Zeyou WANG
;
Yongliang YAO
;
Jianhong WU
;
Guangxin LI
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Macrophage;
Inflammatory response
- From:
Chinese Journal of Immunology
2015;(6):737-740
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the inflammatory responses of macrophages treated with Helicobacter pylori. Methods:Cytokines IL-23,IL-10,TNF-α and IL-8 in cell culture supernatant of macrophages stimulated with Helicobacter pylori were determined by ELISA kits,and the expression of intracellular proteins NOS2 and COX2 in Helicobacter pylori treated macrophages was analyzed by Western blot. Then,the apoptosis of Helicobacter pylori stimulated macrophages was detected by flow cytometry. Results:The secretion of cytokines IL-23,IL-10,TNF-α and IL-8 in the culture supernatant of Helicobacter pylori treated macrophages increased significantly (P<0. 05),and the expression of NOS2 and COX2 was enhanced evidently(P<0. 05). Meanwhile,helicobacter pylori could induce the apoptosis of macrophages obviously ( P<0. 03 ) . Conclusion: The inflammatory responses of macrophages treated with Helicobacter pylori would be promoted to inhibit or kill Helicobacter pylori,furthermore,Helicobacter pylori could induce the apotosis of macropha-ges.
