Effect of glycogen synthase kinase-3β inhibitor TWS119 on proliferation and phenotypic characteristics of human γδT cells in vitro
10.3969/j.issn.1000-484X.2015.06.006
- VernacularTitle:糖原合酶激酶-3β抑制剂TWS119对γδT细胞增殖及表型的影响
- Author:
Yongqiang CHEN
;
Lu ZHENG
;
Junquan LIU
;
Xiaoting Lü
;
Yu ZHOU
;
Ling CHEN
;
Jing XU
;
Zhonghai ZHOU
;
Fuxing CHEN
- Publication Type:Journal Article
- Keywords:
TWS119;
γδT cells;
Proliferation;
Phenotypic
- From:
Chinese Journal of Immunology
2015;(6):748-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of glycogen synthase kinase-3β inhibitor TWS119 on hunman γδT cells growth and phenotypic characteristics. Methods:Using γδT medium to cultivate human peripheral blood γδT cells in vitro. After co-cultured with different concentrations of glycogen synthase kinase-3β( GSK-3β) inhibitor 4, 6-disubstituted pyrrolopyrimidine ( TWS119 ) for indicated time,growth curve and Wnt/β-catenin activation of in each group were determined by CCK-8 and Western blot assays. The CD62L and CD45RA expression theγδT cells were detected using flow cytometry. Results:Wnt/β-catenin pathway ofγδT cells could activate by TWS119. In the first group,TWS119 could upregulate the expression of CD62L and CD45RA in dose dependent manner while inhibit the growth and ratio of γδT cells. In the second group,TWS119 could promote the growth and ratio of γδT cells with the increase in concentration from 0 μmol/L to 4. 0 μmol/L and decreased thereafter. Besides,TWS119 could promote the expression of CD62L in a dose-dependent and had no effect on the CD45RA. Conclusion: Human γδT cells isolated from peripheral blood treated with TWS119 gave rise to two subsets of CD45RA+CD62L+γδT cells and CD62L+γδT cells.
