Reversal effect of RNA interference targeting Fascin1 on migration and invasion of glioma cell line U87 MG
10.3969/j.issn.1000-484X.2015.06.010
- VernacularTitle:RNA干扰Fascin1表达抑制胶质瘤细胞U87 MG的侵袭、转移能力
- Author:
Peidong LI
;
Xinjun WANG
;
Qiao SHAN
;
Yuehui WU
;
Zhen WANG
- Publication Type:Journal Article
- Keywords:
RNA interference;
Fascin1;
Glioma;
U87 MG cell line;
Migration and invasion
- From:
Chinese Journal of Immunology
2015;(6):764-768
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the functional role of Fascin1 and its related molecular mechanisms in migration and invasion capacity of glioma cells,we utilized gene specific small interference RNA of Fascin1 in cell line U87 MG. Methods:Fascin1-siRNA or negative siRNA was transfected into U87 MG cells of control group or experiment group. Transwell method was employed to assess the migration and invasion capacity of glioma cells. Western blot analysis was used to detect the protein expression of Fascin1,pAKT and pSTAT3. The impact of PI3K/AKT pathway and STAT3 pathway on migration and invasion of U87 MG cells was verified,via applying LY294002 and LY294002,which was inhibitor of the two pathways respectively. Results:As compared to control groups,the migration and invasion capacity of transfected glioma cells were attenuated about 52% or 43%(P<0. 05),accompanied with the decreased phos-phorylation of AKT and STAT3. As utilizing the inhibitors of AKT and STAT3,attenuated migration and invasion capacity of U87 MG cells were observed. Conclusion:Down-regulated expression of Fascin1 could suppress the migration and invasion capacity of U87 MG cells by inhibiting the phosphorylation of PI3K/AKT pathway and STAT3 pathway.
