Effect of p38 mitogen-activated protein kinase on expression of matrix metalloproteinase-9 and formation of cerebral edema in rats with cerebral ischemia-reperfusion
- VernacularTitle:p38丝裂原活化蛋白激酶途径对大鼠脑缺血再灌注后脑组织基质金属蛋白酶－9表达及脑水肿形成的影响
- Author:
Guohui LI
;
Yaohui WANG
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinase;
matrix metalloproteinase-9;
cerebral ischemia-reperfusion;
cerebral edema
- From:
Journal of Clinical Neurology
2015;(3):214-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of p 38 mitogen-activated protein kinase ( MARK) on expression of matrix metalloproteinase-9(MMP-9) and formation of cerebral edema in rats with cerebral ischemia-reperfusion. Methods Fifty-four SPF male SD rats were randomly divided into sham operation group ( Sham group ) , ischemia-reperfusion group(I/R group) and p38 inhibitor group(SB group).Middle cerebral artery ischemia-reperfusion model was made by modified line plug method .The neurologic exams were assessed at 24 h after reperfusion , Evans Blue method was used tomeasurement Blood-brain barrier`s permeability,the wet-dry ratio was used to measured to estimate cerebral edema ,Western blot was used to detect the expression of phosphorylation p 38 ( p-p38 ) and MMP-9 in the infarct region .Results Compared with Sham group , I/R group had aggravated neurological deficits ( P<0.05 );compared with the I/R group, SB group had alleviate neurological deficits (P<0.05).Compared with Sham group, blood-brain barrier permeability and brain water content of I /R group were significantly increased ( all P<0.05 );compared with the I/R group, blood-brain barrier permeability and brain water content of SB group were significantly decreased(all P<0.05).Compared with Sham group , the expression of p-p38, MMP-9 in margin of ischemia of I/R group were significantly increased (all P<0.05);compared with the I/R group, the expression of p-p38, MMP-9 in margin of ischemia of SB group were significantly decreased (all P<0.05).Conclusions p38 MAPK involved in the formation of brain edema in rats with cerebral ischemia-reperfusion , and its mechanism may be cerebral ischemia-reperfusion activates p38MAPK,and it make the expression of MMP-9 in margin of ischemia up-regulated,damaging blood-brain barrier permeability ,leading to cerebral edema .
