Expression and biological significance of microRNA-204 in nasopharyngeal carci-noma
10.3969/j.issn.1000-484X.2015.06.023
- VernacularTitle:microRNA-204在鼻咽癌中的表达及生物学意义研究
- Author:
Chengyi JIANG
;
Hongtao WANG
;
Lei ZHOU
;
Tao JIANG
;
Yajia XU
- Publication Type:Journal Article
- Keywords:
MircoRNA-204;
Nasopharyngeal carcinoma;
Bcl-2;
SIRT1
- From:
Chinese Journal of Immunology
2015;(6):822-826
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and biological significance of MicroRNA-204 in nasopharyngeal carcinoma (NPC). Methods: qRT-PCR was applied to detect the relative expression of miR-204 in 43 paired nasopharyngeal carcinoma in comparison to the normal nasal mucosa tissues. Pearson chi-square test was used to analyze the relationship between the miR-204 expression and clinical features. The expressions of Bcl-2 and SIRT1 were measured by immunohistochemistry ( IHC ) , Spearman correlation analysis was used to analyze the relationship between miR-204 and Bcl-2,as well as SIRT1. We then transfected the miR-204 mimics into CNE-2 cells,then the Western blot was used to detect the expression of Bcl-2 and SIRT1,which were considered as the potential targets of miR-204. Results:The relative expressions of miR-204 was significantly downregulated in NPC tissues compared to those of the matched normal tumor-adjacent tissues(P<0. 05). Low expression of miR-204 was significantly associated with lymphatic metastasis(P<0. 05) and advanced TNM stage(Ⅲ+Ⅳ,P<0. 05). The expressions of Bcl-2 and Sirt1 in lower miR-204 level group were both higher than in higher miR-204 level group ( P<0. 05 ) . Both the mRNA and protin expression in CNE-2 cells were down-regulated after transfection. Conclusion: Low expression of miR-204 is related to the malignant clinicopathological features in NPC tissues,and miR-204 may through down-regulate Bcl-2 and SIRT1 to suppress NPC genesis and development.
