Effect of Aβ1-42 injection on hippocampus cells in rats and protective role of polygona-polysaccharose for Alzheimer’s disease
10.11817/j.issn.1672-7347.2014.04.004
- VernacularTitle:Aβ1-42海马注射对大鼠海马细胞的影响及黄精多糖的干预研究
- Author:
Yuxin YI
;
Shixing WU
;
Maosheng YE
;
Yi ZENG
;
Ping ZHANG
;
Yiqun XIE
- Publication Type:Journal Article
- Keywords:
Alzheimer’s disease;
polygona-polysaccharose;
β-amyloid peptide;
cognitive handicap
- From:
Journal of Central South University(Medical Sciences)
2014;(4):344-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the effect of polygona-polysaccharose (PP) on learning and memory ability in rats with Alzheimer’s disease (AD). Methods: Forty ifve Sprague-Dawley rats were assigned into 3 groups. Rats in the sham-operated group were injected with normal saline. Rats in the Aβ group were injected with Aβ1-42. Rats in the PP group were injected with 16% PP solution for 45 days consecutively. hTe Morris water maze was used to investigate the ability of learning and memory in the rats. hTe effect of Aβ and PP on the hippocampus cells was observed by HE and Congo red staining of methanol. Results: Rats in the sham-operated group had no obvious morphological change; and morphology of rats in the PP group was basicaly normal. The layer of pyramidal cells in the Aβ group was decreased. hTe cells appeared sparse and irregular and became smaller. Karyopyknosis and vacuolardegeneration cells were also found. More positive staining materials aggradated in the Aβ group compared with the PP group by Congo red staining (P<0.05). Conclusion: Aβ infusion into the hippocampus results in the impairment of the neuronal degeneration in the rats, which shows similar characterizations of AD. PP can reduce the deposition of Aβ in the hippocampus.
