Effect of piperine on metablism and distribution of nortriptyline in mice
10.11817/j.issn.1672-7347.2014.04.005
- VernacularTitle:不同剂量的胡椒碱对去甲替林在小鼠脑血分布中的影响
- Author:
Lijing CAI
;
Jiawen LIU
;
Lanlin LI
;
Shaolin YANG
;
Jian YANG
;
Qizhi ZHANG
;
Wenxing PENG
- Publication Type:Journal Article
- Keywords:
piperine;
nortriptyline;
P-glycoprotein;
blood-brain barrier;
pharmacokinetics
- From:
Journal of Central South University(Medical Sciences)
2014;(4):349-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the pharmacokinetics and brain/plasma concentration ratio of nortriptyline at multiple doses in mice which were pre-treated with physiological saline, piperine and verapamil. Methods: A total of 216 male Kun Ming mice[(25±3) g] were equally divided into 4 groups randomly. Each group was intragastrically administered physiological saline (B), piperine (170 μg/kg), piperine (5 mg/kg) and verapamil (5 mg/kg) for 8 days. On the 8th day, 1 h atfer giving the above drugs, each mice was intraperitoneally injected nortriptyline (13 mg/kg). The mice were sacriifced by picking off eyeballs at the time intervals of 5, 15, 30 min, and 1, 2, 4, 6, 8 and 12 h, andthe cerebra were collected and weighted. Nortriptyline in mouse plasma and brain was determined by HPLC-MS/MS. The pharmacokinetic properties of the plasma, brain and brain/plasma were calculated. Results: hTe AUC0-12 h of brain/plasma concentration ratio in the 170 μg/kg piperine group was significantly lower than that in the other groups (P<0.05), while the AUC0-12 h of brain/plasma concentration ratios in the 5 mg/kg piperine group and the verapamil group were not signiifcantly different from those of untreated mice. Conclusion: Piperine (170 μg/kg) may induce P-glycoprotein expression in the blood-brain barrier, while piperines at 5 mg/kg has no influence on P-glycoprotein expression in the blood-brain barrier.
