Ketamine induces apoptosis of human uroepithelial SV-HUC-1 cells

VernacularTitle:氯胺酮诱导人膀胱SV-HUC-1细胞凋亡的实验研究
Author: Liang HUANG ; Zhengyan TANG ; Dongjie LI ; Guilin WANG ; Ke HUANG ; Jie ZHOU ; Yonglin LI ; Benmin GAO ; Junjie CHEN
Publication Type:Journal Article
From: Journal of Central South University(Medical Sciences) 2014;(7):703-707
CountryChina
Language:Chinese
Abstract: Objective:To determine the effect of ketamine on the apoptosis of human uroepithelial cells (SV-HUC-1) and the pathogenesis of ketamine-associated cystitis. Methods:SV-HUC-1 cells were cultured under various concentrations of ketamine and different time. Flow cytometry was used to analyze the rate of cell apoptosis. hTe protein levels of Bax, Bcl-2, pro-caspase-3, and cleaved caspase-3 were detected by Western blot. Results:Compared with the control group, the apoptotic rate of ketamine cultured SV-HUC-1 cells increased. hTe expression of Bax increased, Bcl-2 expression decreased, and Bax/Bcl-2 in the ketamine cultured SV-HUC-1 cells was signiifcantly higher. hTe protein level of pro-caspase-3 was signiifcantly lower, and that of cleaved caspase-3 was signiifcantly higher than that in the control group (P<0.05), positively correlated with the dose of ketamine and time of culture (P<0.05). Conclusion:Ketamine can induce the apoptosis of SV-HUC-1 cells in a dose and time dependent manner.