Expression profiles of genes in wild-type DJ-1 and A39S DJ-1 mutant cells
10.11817/j.issn.1672-7347.2014.04.001
- VernacularTitle:野生型DJ-1与A39S突变型DJ-1表达的单克隆细胞株基因表达谱分析
- Author:
Zhenhua LIU
;
Beisha TANG
;
Dan HE
;
Lei WANG
;
Zhiquan XIAO
;
Xinxiang YAN
;
Jifeng GUO
- Publication Type:Journal Article
- Keywords:
DJ-1;
A39S mutation;
expression proifle of gene;
DNA microarray;
UGT2B7 gene
- From:
Journal of Central South University(Medical Sciences)
2014;(4):325-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To elucidate the role of A39S mutation of DJ-1 in the onset of Parkinson’s disease (PD) and identify genes for which expressions are abnormally regulated by A39S DJ-1 mutation. Methods: We established HEK293 cell lines which stably expressed empty vector, wild-type DJ-1 and A39S mutated DJ-1 respectively. DNA microarrays were used to identify genes for which expressions change in wild-type DJ-1 cells and A39S DJ-1 mutant cells. Results: Compared with the cell line expression empty vector, we identified 42 differentially regulated genes (including 14 up-regulated genes and 28 down-regulated genes) in the wild-type DJ-1 cells and 8 differentially regulated genes (including 6 up-regulated genes and 2 down-regulated genes) in the A39S DJ-1 mutant cells. Compared with the wild-type DJ-1 cells, only the expression of UGT2B7 gene was down-regulated in A39S DJ-1 mutant cells. hTese differentially regulated genes were mainly related to signal transduction, regulation of transcription, apoptosis and metabolism. Conclusion: A39S mutated DJ-1 may disturb the transcriptional activities of DJ-l and involve in the pathogenesis of PD.
