Effect of Zibai Gelatin for Cervical Cancer SiHa Cells
10.3969/j.issn.1005-5304.2015.07.017
- VernacularTitle:紫柏凝胶对宫颈癌SiHa细胞的影响
- Author:
Kai XU
;
Xiaoou XUE
;
Jian LI
;
Xiuli MA
;
Lei QIN
- Publication Type:Journal Article
- Keywords:
HPV;
medicated serum;
Zibai Gelatin;
immunologic function
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(7):60-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunological reaction mechanism of medicated serum in Zibai Gelatin for SiHa cells of the cervical cancer infected by high risk human papilloma virus (HPV). Methods Through immunohistochemical comparison and the cell culture, and after medicated serum was administrated to SiHa cells of cervical cancer for 24 h, 48 h, and 72 h, optical densities of IL-6, IL-10, CD83 and TNF-αin the same time but different concentrations and different times but the same concentration were observed. Relationships of dose-effect and time-effect between expressions of IL-6, IL-10, CD83 and TNF-α and medicine action were analyzed by calculating average optical density. Results With the increase of medicine concentration and administration time of Zibai Gelatin, the expressions of IL-6 and TNF-αgradually decreased, while the expressions of CD83 and IL-10 gradually increased (P<0.05). Conclusion Zibai Gelatin with medicated serum can inhibit local inflammatory reaction by improving local immunologic function of cervix, which is beneficial to reduce cervix high-risk HPV.
