Analysis on Isatidis Radix Protein in Different Areas by SDS-PAGE
10.3969/j.issn.1005-5304.2015.07.024
- VernacularTitle:不同地区板蓝根蛋白SDS-PAGE分析
- Author:
Xin YANG
;
Qiuhong WANG
;
Yue WANG
;
Hui GUO
;
Na XING
;
Haixue KUANG
- Publication Type:Journal Article
- Keywords:
Isatidis Radix;
identify;
SDS-PAGE
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(7):86-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify Isatidis Radix in different areas by SDS-PAGE;To provide the basis for the identification and quality evaluation of Isatidis Radix. Methods By using SDS-PAGE technology, the protein profiles of Isatidis Radix in Heilongjiang and Hebei regions were established. Results Isatidis Radix protein in Heilongjiang contained about 13 protein subunits, and the major subunits were 10.5 kD, 23.0 kD, 24.9 kD, and 44.1 kD. Isatidis Radix protein in Hebei contained about 12 subunits, and the major subunits were 55.6 kD, 45.9 kD, 34.3 kD, 18.9 kD, 12.4 kD, and 10.5 kD. Conclusion SDS-PAGE technology can be used as one of the references to identify Isatidis Radix in different areas.
