Simultaneous Determination of Four Components in Huangqi Shengmai Decoction by HPLC-DAD

VernacularTitle:HPLC-DAD同时测定黄芪生脉饮中4种成分含量
Author: Cheng LU ; Shenghui ZHOU ; Wei LI ; Xiuli ZHENG ; Qingjian GAO ; Hao TANG
Publication Type:Journal Article
Keywords: Huangqi Shengmai Decoction; schisandrin B; schisantherin A; lobetyolin; ruscogenin; HPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;(7):96-99
CountryChina
Language:Chinese
Abstract: Objective To establish a method for simultaneous determination of contents of schisandrin B, schisantherin A, lobetyolin and ruscogenin in Huangqi Shengmai Decoction with HPLC method. Methods The chromatographic column was an Agilent Eclipse C18 column (4.6 mm × 250 mm, 5 μm) that was maintained at a constant temperature of 30 ℃. The mobile phase was 0.1% acetic acid solution-acetonitrile, with gradient elution. The flow rate was 1.0 mL/min. The detection was at wavelength of 280 nm. Results The standard curves of schisandrin B, schisantherin A, lobetyolin and ruscogenin had good linearity in the ranges of 0.862 5-21.562 5μg (r 2=0.999 1), 0.737 5-18.437 5μg (r 2=0.999 4), 0.095 2-2.380 0 μg (r 2=0.999 6), and 0.810 0-20.250 0 μg (r 2=0.999 0), respectively. The average recoveries of the four components were 98.06%(RSD=1.64%), 99.61%(RSD=2.72%), 97.98%(RSD=1.45%), and 99.30%(RSD=1.25%), respectively. Conclusion This method is accurate, rapid and specific, achieving the stable results with high reproducibility.