Effects of Jianpi Yangxue Qufeng Formula on Aquaporin-3 in Model Mice Skin Tissue with Chronic Eczema
10.3969/j.issn.1005-5304.2015.07.014
- VernacularTitle:健脾养血祛风方对脾虚慢性湿疹模型小鼠皮肤组织水通道蛋白3的影响
- Author:
Jing TIAN
;
Xinhong LI
;
Huiwen ZHU
;
Xiande MA
;
Changqing FENG
;
Min WANG
- Publication Type:Journal Article
- Keywords:
Jianpi Yangxue Qufeng Formula;
spleen deficiency;
chronic eczema;
AQP3;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(7):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Jianpi Yangxue Qufeng Formula (JPYXQF) on the AQP3 in mice with chronic eczema, and explore mechanism of action. Methods Fifty healthy male mice were randomly divided into 5 groups, namely normal group, model group, positive medicine group and JPYXQF high and low dose groups. Low-dose DNCB and Sennae Fominm were used to establish mice models of chronic eczema with spleen deficiency. JPYXQF groups were treated by JPYXQF for gavage, while the positive medicine group was treated by levocetirizine hydrochloride for gavage. The expression of AQP3 in mice skin tissue was detected by immunohistochemical method. At the same time, the pathological changes of skin were observed. Results The pathology of mice skin lesion showed that JPYXQF has certain recovery effects on the inflammation injury of skin lesion. Compared with the normal group, expression of AQP3 over expressed in model group. Compared with the model group, the expression of AQP3 in all treatment groups significantly decreased, and the staining intensity decreased. In the model group, the average optical density of AQP3 was significantly higher than that in the normal group (P<0.01). Compared with the model group, the treatment groups can reduce the expression of AQP3 in mice skin tissues (P<0.05). Conclusion JPYXQF can reduce the over expression of AQP3 in skin lesion, which is probably its mechanism for the treatment of chronic eczema.
