Effects of iPSC-MSCs on mitochondria of PC12 cells injured by CoCl2
10.3969/j.issn.1000-4718.2015.06.013
- VernacularTitle:iPSC－MSCs 对 CoCl2诱导的 PC12细胞损伤时线粒体功能的影响
- Author:
Yan YANG
;
Hui LI
;
Zhanpeng SUN
;
Chunlin HU
;
Xiaoli JING
;
Hongyan WEI
;
Xiaoxing LIAO
;
Xin LI
- Publication Type:Journal Article
- Keywords:
Cobalt chloride;
PC12 cells;
Apoptosis;
Induced pluripotent stem cells;
Mitochondria
- From:
Chinese Journal of Pathophysiology
2015;(6):1036-1041
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effects of induced pluripotent stem cells-derived mesenchymal stem cells ( iPSC-MSCs) on cobalt chloride ( CoCl2 )-induced injuries of PC12 cells and its possible mechanism.METHODS:PC12 cells were exposed to CoCl2 to set up a chemical-induced cellular injury model and were cocultured with iPSC-MSCs.The cell viability was tested by CCK-8 assay.The apoptosis was measured by flow cytometry using Annexin V/PI staining.The mitochondrial membrane potential (MMP) was analyzed by flow cytometry using JC-1 staining.Immunofluorescence was employed to observe mitochondrial transfer from iPSC-MSCs to PC12 cells.RESULTS: Apoptosis of PC12 cells was in-creased and MMP of PC12 cells was decreased after exposed to CoCl2 at concentration of 400μmol/L for 24 h.Coculture of PC12 cells with iPSC-MSCs reduced the apoptosis and recovered the MMP of the PC12 cells.Tunneling nanotubes were formed between iPSC-MSCs and PC12 cells, through which the iPSC-MSCs transferred the mitochondria to the PC12 cells. CONCLUSION:iPSC-MSCs protect PC12 cells from CoCl2-induced injuries, which may be associated with the mitochon-drial transfer from iPSC-MSCs to PC12 cells.
