Proteins are transfected into bone marrow mesenchymal stem cells by polyethyleneimine-protein nano-complexes
10.3969/j.issn.1000-4718.2015.06.016
- VernacularTitle:聚乙烯亚胺－蛋白纳米复合体转染蛋白进入骨髓间充质干细胞的应用研究
- Author:
Lin JIANG
;
Yueheng WU
;
Xiaohong LI
;
Yu PAN
;
Jing XIAO
;
Xiangyu YANG
;
Yuan FENG
;
Xiyong YU
- Publication Type:Journal Article
- Keywords:
Polyethyleneimine;
Bone marrow mesenchymal stem cells;
Protein transfection
- From:
Chinese Journal of Pathophysiology
2015;(6):1057-1063
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the role of encapsulated protein transfected into human bone marrow mesen-chymal stem cells ( hBMSCs) by polyethyleneimine ( PEI) , and to optimize the best mole ratio of PEI-proteins.METH-ODS:6 groups of DNase I-PEI complexes were constructed and the best mole ratio was explored by laser scattering analy-sis.The appearance of complexes was presented under transmission electron microscope.Meanwhile, 4 groups of construc-ted GFP-PEI complexes were utilized to transfect into the hBMSCs, which were isolated and expand in vitro.The fluores-cence intensity of transfected cells was observed under confocal microscope.In addition, the cytotoxicity of the complexes on the cell proliferation was detected by MTT assay.The activity of the intracellular proteins was testified by aβ-galactosi-dase staining experiment.RESULTS:When the mole ratio of PEI and protein was adjusted to 4∶1, the complex transfec-tion efficiency was the best, and β-galactosidase color test turned blue.CONCLUSION:PEI has the character of encap-sulating various proteins to nano-complexes.The proteins transfected into bone marrow mesenchymal stem cells are con-firmed to have functional activity.As a protein carrier, PEI is of high efficiency and low toxicity, thus providing a new way for stem cell reprogramming.
