Development of cell model of polymer/liquid crystal and effect of their elasticity on adhesion of rBM-MSCs
10.3969/j.issn.1000-4718.2015.06.017
- VernacularTitle:聚合物／液晶细胞模型的构建及其表面弹性对大鼠骨髓干细胞黏附的影响
- Author:
Yanshan GUO
;
Hao WU
;
Liheng ZHENG
;
Yupan SHANG
;
Mei TU
;
Jiaqing ZHANG
- Publication Type:Journal Article
- Keywords:
Cell model of polymer/liquid crystal;
Rat bone marrow mesenchymal stem cells;
Cytoskeleton staining
- From:
Chinese Journal of Pathophysiology
2015;(6):1064-1069
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To develop the cell model of polymer/liquid crystal and to study the effect of their elasticity on the adhesion of rat bone marrow mesenchymal stem cells (rBM-MSCs).METHODS: Using the method of solvent e-vaporation induced phase separation, the cell model of polymer/liquid crystal was constructed.The surface morphology and phase separation structure were determined by polarized optical microscopy ( POM) , scanning electron microscopy ( SEM) and small angle X-ray scattering ( SAXS ) .rBM-MSCs were separated and expanded by adherent culture.The surface markers of rBM-MSCs were detected by flow cytometry.The cells were induced to osteogenic differentiation and adipogenic differentiation for 2 weeks.After 3 passages, the cells were divided into 4 groups, including total PU control group, 10%membrane group, 30%membrane group and 50%membrane group.The cells were then incubated with rhodamine phalloi-din for cytoskeleton staining and were observed under the confocal laser scanning microscope after cultured for 24 h.RE-SULTS:The cell model of polymer/liquid crystal was constructed successfully using the method of solvent evaporation in-duced phase separation.Flow cytometry results showed that the rBM-MSCs positively expressed CD29, CD44 and CD90, and negatively expressed CD34 and CD45.After stained with alizarin red S and oil red O, the calcium nodule and lipid droplets in rBM-MSCs were observed obviously.The cytoskeleton staining result indicated that the area in total PU control group, 10%membrane group and 30%membrane group were greater, and the actin microfilaments were also clearer than
that in 50%membrane group.CONCLUSION:The cell model with suitable content of liquid crystal made a contribution to the rBM-MSCs’ adhesion, but too much liquid crystal inhibits cell adhesion.
