Injury effect of recombinant soluble human CD40 ligand on human um-bilical vein endothelial cells
10.3969/j.issn.1000-4718.2015.06.025
- VernacularTitle:重组可溶性人 CD40L 诱导人脐静脉内皮细胞损伤
- Author:
Hongli LIU
;
Meng CHEN
;
Hongtao WANG
;
Yiling WU
- Publication Type:Journal Article
- Keywords:
Recombinant soluble human CD40 ligand;
Human umbilical vein endothelial cells;
Atherosclerosis
- From:
Chinese Journal of Pathophysiology
2015;(6):1111-1114
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the damage in human umbilical vein endothelial cells ( HUVECs) induced by recombinant soluble human CD40 ligand (rshCD40L).METHODS:The cultured HUVECs were treated with rshCD40L for 12 h.The survival activity of the HUVECs was observed by MTS assay.The expression of E-selectin, intercellular ad-hesion molecule (ICAM)-1, tissue factor (TF) and tissue factor pathway inhibitor (TFPI) was measured by ELISA.The activity of superoxide dismutase ( SOD) and the level of malondialdehyde ( MDA) were detected by the methods of thibabi-turic acid (TBA) .RESULTS:Compared with normal group, different concentrations of rshCD40L (0.5, 1, 2, 3 mg/L) had no obvious effect on the survival activity of the HUVECs (P>0.05).rshCD40L at concentration of 0.5 mg/L promo-ted the secretion of E-selectin, sICAM-1, TF and TFPI in the HUVECs (P<0.01).rshCD40L at concentration of 0.5 mg/L also increased MDA content and reduced the activity of SOD in the HUVECs (P<0.05).CONCLUSION:0.5~3mg/L rshCD40L has no obvious effect on endothelial cell survival, but already causes endothelial dysfunction by increas-ing endothelial inflammation and exogenous coagulation reaction, inducing lipid peroxides injury and reducing antioxidant capacity.
