MSC-conditioned medium activates Nrf2/ARE pathway to protect H9c2 cells against oxidative stress
10.3969/j.issn.1000-4718.2015.06.001
- VernacularTitle:间充质干细胞条件培养基激活 Nrf2／ARE 通路对抗 H2 O2致 H9 c2细胞的氧化应激损伤
- Author:
Xi DONG
;
Guibo SUN
;
Yun LUO
;
Suhong CHEN
;
Xiaobo SUN
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Conditioned medium;
Myocardial cells;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2015;(6):961-966
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM: To investigate the protective effect of mesenchymal stem cell ( MSC)-conditioned medium (MSCCM) on myocardial cell line H9c2 and its mechanism.METHODS:Verification of MSC was performed by flow cy-tometry analysis, followed by MTT assay to determine the optimal incubation time of MSCCM with myocardial cells.The cells were divided into 4 groups:normal ( N) group, model ( M) group, M+MSCCM group and MSCCM group.The cells in M+MSCCM group and MSCCM group were pre-incubated with MSCCM for 24 h.The cells in M group and M+MSCCM group were treated with 300 μmol/L H2 O2 for 4 h to imitate oxidative injury of myocardial cells.Mitochondrial membrane potential and apoptotic rate of injured myocardial cells were detected by flow cytometry.The ROS production was measured by fluorescence microscopy.The nuclear translocation of Nrf2 and expression of HO-1 was examined by Western blot.RE-SULTS:No difference of mitochondrial membrane potential, apoptotic rate or ROS production between MSCCM group and N group was observed (P>0.05).The mitochondrial membrane potential depolarization, apoptotic rate and ROS produc-tion in M+MSCCM group were significantly lower than those in M group ( P<0.01 ) .The nuclear translocation of Nrf2 and expression of HO-1 in the myocardial cells were increased with MSCCM incubation time prolonged.CONCLUSION:MSCCM protects the myocardial cells against oxidative injury induced by H2 O2 .The anti-oxidative mechanism would be as-sociated with the activation of Nrf2/ARE pathway.
