Antioxidant Assay of Extracted Fractions Xanthium Strumarium L Using Lens Protein Crosslink Activity.
- Author:
Seok Jong LEE
1
;
Kwang Won LEE
;
Young Shin CHUNG
;
Eun Kyung HONG
;
Jin Hak LEE
;
Won Rayng WEE
;
Choun Ki JOO
Author Information
1. Laboratory of Ophthalmology and Visual Science, College of Medicine, The Catholic university of Korea, Seoul, Korea. ckjoo@cmc.cuk.ac.kr
- Publication Type:Original Article
- Keywords:
Cataract;
Crosslink;
Glycation;
Antioxidant
- MeSH:
Antioxidants;
Cataract;
Chromatography, Ion Exchange;
Epithelial Cells;
Humans;
Xanthium*
- From:Journal of the Korean Ophthalmological Society
2002;43(4):751-756
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To elucidate the anti-oxidant effect of extract fractions from Xanthium strumarium L. on lens protein by crosslinking assay. METHODS: [(1 4)C] N-formyl-lysine was synthesized and purified by ion exchange chromatography. The crosslinking activities of extract fractions(Xan Crude, Xan CHCl3, Xan EtAc and Xan H2O) to lens protein were determined by incorporation with [(14)C] N-formyl-lysine. RESULTS: It was observed that Xan Crude, Xan CHCl3, and Xan EtAc extracted from Xanthium strumarium L. showed approximately 10% of antioxidant effect whereas Xan H2O showed no effect by crosslinking assay. CONCLUSIONS: This study showed that the crosslinking assay described in this study can be developed as a potential tool to screen the anti-oxidant effect rapidly and accurately compared to MTT assay. The result was compared to MTT assay using Human Lens epithelial cell line.
