Role of up-regulated microRNA145 in viability, apoptosis, invasion and metastasis of hepatoma cells
10.3969/j.issn.1000-4718.2015.06.010
- VernacularTitle:上调 miRNA145对肝癌细胞的作用及其机制研究
- Author:
Huanyu WANG
;
Yafeng WANG
;
Kunsong ZHANG
;
Chaohui ZHANG
;
Zijian ZHANG
;
Shanzhou HUANG
;
Jian WU
;
Baogang PENG
;
Dong CHEN
;
Qi ZHOU
- Publication Type:Journal Article
- Keywords:
MicroRNA145;
Hepatocellular carcinoma;
Cell proliferation;
Apoptosis;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2015;(6):1019-1025
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effects of microRNA145 ( miRNA145 ) on the viability, apoptosis, inva-sion and metastasis of hepatoma HepG2 cells.METHODS: HepG2 cells were randomly allocated into 3 groups: blank control group, empty mimic transfected group and miRNA145 mimic transfected group.Under the induction of Lipofectami-neTM 2000, the recombinant was transfected into HepG2 cells.After transfection, the expression level of miRNA145 was detected by real-time PCR.The protein level of N-cadherin and the mRNA expression levels of miRNA145 and N-cadherin were detected by Western blot and real-time PCR.The cell viability was detected by MTS assay.The cell cycle and apopto-sis were analyzed by flow cytometry.Invasion and metastasis were detected by Transwell assay.RESULTS:Compared with negative control, miRNA145 expression was up-regulated significantly, while the expression of N-cadherin was down-regu-lated significantly.Meanwhile, the cell viability, cell cycle, apoptosis, invasion and metastasis of hepatoma HepG2 cells were all significantly inhibited (P<0.05).CONCLUSION:miRNA145 dramatically inhibits viability, apoptosis, inva-sion and metastasis of hepatoma cells.
