Effects of maternal limb ischemic preconditioning on structural and func-tional changes of mitochondria in fetal hippocampal neurons induced by intrauterine distress-reoxygenation in rats
10.3969/j.issn.1000-4718.2015.06.027
- VernacularTitle:母体肢体缺血预处理对宫内窘迫胎鼠复氧后海马神经元线粒体结构和功能的影响
- Author:
Huan LU
;
Xiaochun ZHENG
;
Xiaolin CHEN
;
Xizhu WU
;
Guanlin ZHENG
- Publication Type:Journal Article
- Keywords:
Limb ischemia preconditioning;
Fetal hypoxia/reoxygenation injury;
Mitochondria
- From:
Chinese Journal of Pathophysiology
2015;(6):1120-1124
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effects of maternal limb ischemic preconditioning ( LIP) on the mitochon-drial structures and functions of the hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats. METHODS:Pregnant rats (n=40) were randomly divided into 4 groups: sham (S) group, LIP group, fetal distress ( FD) group and LIP+FD group.Intrauterine ischemia model was established through the experimental design.The ultra-structure of the mitochondria in CA1 area of the hippocampus was observed .The mitochondrial membrane potential and re-active oxygen species ( ROS) were measured .The content of ATP and MDA in the hippocampus tissue was detected.The activity of Mn-SOD was observed.RESULTS:Compared with sham group, the ultrastructure of mitochondria in CA1 area of the hippocampus was damaged in FD group and LIP+FD group.The mitochondrial membrane potential, the content of ATP and the activity of Mn-SOD were decreased.However, the content of ROS and MDA was increased.Compared with FD group, the ultrastructure of mitochondria in CA1 area of the hippocampus was intact in LIP+FD group.Furthermore, the reduced mitochondrial membrane potential and ATP content were inhibited.The activity of Mn-SOD was increased, but the content of ROS and MDA was decreased in LIP+FD group.CONCLUSION:Limb ischemia preconditioning inhibits the damage the mitochondria of fetal hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats.
