Recombinant expression of Schistosoma japonicum fructose-1,6-bisphos-phate aldolase and its expression in different developmental stages of S. ja-ponicum
10.16250/j.32.1374.2015010
- VernacularTitle:日本血吸虫果糖二磷酸醛缩酶的重组表达及其在血吸虫生活史各阶段的表达分析
- Author:
Ke YAN
;
Zhengrong ZHONG
;
Yunxia XU
;
Shuqin DING
;
Jianguo HU
;
Yuanhong XU
;
Qingli LUO
;
Jilong SHEN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Fructose-1 6-bisphosphate aldolase;
Cloning;
Expression;
Purification
- From:
Chinese Journal of Schistosomiasis Control
2015;(3):277-281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone express and purify Schistosoma japonicum fructose?1 6?bisphosphate aldolase SjFBPA in E. coli and observe its expression in different developmental stages of S. japonicum. Methods FBPA gene was amplified from S. japonicum adult worm cDNA by using PCR. The amplified product was recombined into pET28a plasmid and inducibly expressed with IPTG in E. coli BL21. SDS?PAGE and Western blotting were employed to analyze and identify the recombinant protein SjFBPA rSjFBPA . Then rSjFBPA was purified by chromatographic purification and its purity was analyzed by SDS?PAGE. The protein concentration of rSjFBPA purified was measured by the BCA method. Furthermore SjFBPA mRNA was ana?lyzed in different developmental stages of S. japonicum by RT?PCR. Results SjFBPA was successfully amplified by using PCR and identified by restriction enzyme digestion and sequencing. The Western blotting analysis confirmed that the recombinant pro?tein could specifically reactive to the anti?His?tag monoclonal antibody. The concentration of the purified recombinant protein was about 4 mg/ml. The result of RT?PCR showed that SjFBPA mRNA was expressed in cercaria schistosomulum adult worm and egg of S. japonicum. Conclusion SjFBPA is successfully recombined and expressed in a prokaryotic system and SjFBPA mRNA is expressed in cercaria schistosomulum adult worm and egg of S. japonicum.
