Introduce a improved method for the production of hemoglobin liquid by use of the hemoglobin electrophoresis alkaline
10.3969/j.issn.1673-4130.2015.12.046
- VernacularTitle:1种血红蛋白电泳液标本改良方法的探讨
- Author:
Ling ZHANG
;
Zhaohui HU
;
Zhouping GUO
;
Xiaodong LIN
;
Jianhua PAN
;
Qingyi ZHU
- Publication Type:Journal Article
- Keywords:
hemoglobin liquid;
preparation method;
toxic reagent
- From:
International Journal of Laboratory Medicine
2015;(12):1746-1748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To introduce a improved method for the production of hemoglobin liquid by use of the hemoglobin electro‐phoresis alkaline .Methods First ,we used the pipette to absorb the settlement of red blood cells from a batch of EDTA anticoagula‐ted whole blood specimens ,then dropped them into the 0 .9% saline washed human erythrocytes .With the help of the pipette nozzle we pipet from the liquid surface to bottom repeatedly ,made the red cells suspended in the liquid evenly .The samples should be cen‐trifuged and the red blood cells fully deposited at the bottom ,then poured the supernatant after centrifugation to remove the impuri‐ties in plasma and leave the allowance of red blood cells .We add distilled water or hemolysin to lysis RBC .Hands up test tubes rack using wrist gently back and forth several times until the hemolysis was clear and transparent .Results Compared the results of the modified method and the traditional method ,then the two results compared with the results of HPLC recommended by international association of thalassemia method .Compared the results of three screening methods with that of the thalassemia gene identification method .So we could objectively evaluate the reliability of the test .Conclusion The result of the improved method are same to the electrophoretogram by SOP operation ,it will be more efficient and reduce the cost of reagent .The whole process of preparing hemo‐globin solution doesn′t contact with any chemical reagent .So there will be no pollution to the environment ,and it also reduces the harmful of toxic reagent to the human body .
