Comparison of effects of ELISPOTs using pleural fluid and peripheral blood in the diagnosis of tuberculous pleurisy
10.11958/j.issn.0253-9896.2015.06.028
- VernacularTitle:胸腔积液与外周血ELISPOT检测诊断结核性胸膜炎的效果比较
- Author:
Dan CUI
;
Jie ZHAO
;
Pengchong LIU
;
Xiuli FENG
;
Zhihui LI
- Publication Type:Journal Article
- Keywords:
enzyme-linked immunospot assay;
spot forming cells;
pleural effusion mononuclear cells;
peripheral blood mononuclear cells
- From:
Tianjin Medical Journal
2015;(6):681-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of ELISPOT (enzyme-link immunospot) test using different samples in diagnosing tuberculous pleurisy. Methods Using T-Spot-TB kit to detect interferon-γlevel in pleural effusion and periph?eral blood from 164 patients with tuberculous pleural effusion and 102 patients without tuberculous pleural effusion. Number of spot forming cells (SFCs) as well as the specificity and sensitivity of the tests were compared between these two methods (ELISPOT using leural effusion or peripheral blood). Results The area under the ROC curve was 0.947 in pleural effusion Elispot test while it was 0.905 in peripheral blood Elispot test. The sensitivity of pleural effusion ELISPOT test in diagnosis of tuberculous pleurisy (95.1%) was significantly higher than that of peripheral blood ELISPOT test (89.0%). What’s more, the specificity of pleural effusion ELISPOT test in diagnosis of tuberculous pleurisy (90.2%) was higher than that in diagno?sis of peripheral blood ELISPOT test (88.2%). Conclusion The pleural effusion ELISPOT test is more valuable than periph?eral blood ELISPOT in the diagnosis of tuberculous pleuritis.
