Isolation of rat bone marrow-derived mesenchymal stem cells and explore its role in immunomodulation
10.11958/j.issn.0253-9896.2015.06.009
- VernacularTitle:大鼠骨髓间充质干细胞的分离鉴定及免疫调节性能初探
- Author:
Zhiqiang ZHANG
;
Yi LIU
;
Keqiu LI
;
Guang LI
- Publication Type:Journal Article
- Keywords:
bone marrow;
mesenchymal stem cells;
cell differentiation;
neuroimmunomodulation;
rats,Sprague-Daw-ley;
rats,Wistar
- From:
Tianjin Medical Journal
2015;(6):611-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the immunomodulation property of bone marrow-derived mesenchymal stem cells (BMSCs) from Sprague-Dawley (SD) rats after they are isolated, cultured and identified by surface marker and differentiation potential examination. Methods BMSCs were isolated from femur and tibia of SD rats and passaged by trypsinization. The surface markers of the 3rd passage BMSCs were detected by flow cytometry and the capacity of their adipocyte and cartilage differentiation were examined. In order to explore the immunomodulation property of BMSCs, allogeneic spleen T cells of Wi?star rats were co-cultured with BMSCs through either cell-to-cell contact or transwell, then its effect on the T cell subsets and related mechanism was also examined. Results BMSCs were mainly spindle-shaped in culture. Surface marker detec?tion showed that BMSCs expressed high levels of CD29, CD44 and CD90 but no CD34 nor CD45 at the third generation. Un?der specific condition, BMSCs could differentiate into adipocytes and chondrocytes. The CD8+effector T cells (Teffs) decreas?es effectively and the CD4+CD25+regulatory T cells (Tregs) increased remarkably when BMSCs were co-cultured with allo?geneic spleen T cells for 48 hours. The expressions of IL-10 and TGF-β1 of BMSCs significantly increased after co-culture with T cells, and this effect was more obvious in cell-to-cell contact group. Conclusion The immunomodulation property of BMSCs were presumably function through cell-to-cell contacts and cytokine secretion.
