Determination of Ceftazidime for Injection by HPLC
10.3870/yydb.2015.06.027
- VernacularTitle:高效液相色谱法测定注射用头孢他啶聚合物
- Author:
Yingqian LIU
;
Qun WU
;
Lijun LUO
;
Qian TANG
- Publication Type:Journal Article
- Keywords:
Ceftazidime for injection;
Polymer;
Chromatography,high performance liquid
- From:
Herald of Medicine
2015;(6):802-804
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the determination of ceftazidime for injection. Methods HPLC column was TSK-GEL G2000SWXL gel column. Mobile phase of phosphate buffer ( pH 7. 0) was 0. 005 mol · L-1 disodium hydrogen phosphate solution-0. 005 mol · L-1 sodium dihydrogen phosphate solution ( 61 : 39 ). The detection wavelength was 231 nm; column temperature was 30 ℃ ; the injection volume was 20 μL. Results Ceftazidime reference linear range was 0. 51-25. 64 μg·mL-1(r=0. 999), ceftazidime for injection polymer linear range was 0. 20-3. 92 mg·mL-1 (r=0. 99), and the limit of quantification polymer was 0. 71 μg. Conclusion The method is rapid and the separation was good. It can be used for the detection of ceftazidime for injection.
