Comparison of ELISA and colloidal gold immunochromatographic assay for detection of serum anti-CCP antibody in the diagnosis of RA
10.3969/j.issn.1673-4130.2015.11.020
- VernacularTitle:ELISA 与胶体金免疫层析法检测血清抗 CCP 抗体在 RA 诊断中的比较
- Author:
Weihua TIAN
;
Wenyuan MA
;
Shasha LI
- Publication Type:Journal Article
- Keywords:
enzyme-linked immunosorbent assay;
anti-cyclic citrullinated peptide antibody;
rheumatoid arthritis
- From:
International Journal of Laboratory Medicine
2015;(11):1528-1529
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic values of enzyme‐linked immunosorbent assay (ELISA ) and colloidal gold im‐munochromatographic assay in detecting serum anti‐cyclic citrullinated peptide(anti‐CCP) antibody .Methods Colloidal gold immu‐nochromatographic assay and ELISA were used to detect serum anti‐CCP antibody in 110 RA patients and 110 healthy cases . Results The data were analysed with the paired Chi‐Square Test (χ2 = 174 .354 ,P < 0 .01) ,the difference was significant .The K ap pa value was 0 .890(P< 0 .01) ,indicating that the coincidence between the two kinds of methods was good .The sensitivity , speciality of ELISA were 83 .64% and 92 .73% respectively .Conclusion In detecting serum anti‐CCP Antibody ,there was dife‐rence between colloidal gold immunochromatographic assay and ELISA according to statistics analysis .The sensitivity and speciality of ELISA were higher than those of colloidal gold immunochromatographic assay .
