Determination of (R,S)- Epigoitrin in Banlangen Granules by HPLC
10.3870/yydb.2015.06.024
- VernacularTitle:高效液相色谱法测定板蓝根颗粒中（R,S）-告依春的含量
- Author:
Wei GONG
;
Zhongliang LIU
;
Qinghua ZHAO
;
Fenge WEN
;
Peng LI
;
Kun ZHANG
;
Mei ZHAO
- Publication Type:Journal Article
- Keywords:
Banlangen Granules;
(R,S)-epigoitrin;
Content limitation;
Chromatography,high performance liquid
- From:
Herald of Medicine
2015;(6):791-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC method for determination of (R,S)-epigoitrin in Banlangen granules and discuss the content limitation. Methods The samples were separated on SHIMADZU VP-ODS (150 mm×4. 6 mm, 5 μm) and the mobile phase consisted of methol-0. 02% phosphoric acid solution (7:93) at a flow rate of 1. 0 mL. min-1 . The detection wavelength was set at 245 nm and the injection volume (in automatic sampler) was 10 μL. The content limitation was assessed according to the transfer rate and statistical data of the results. Results (R,S)-epigoitrin showed a good linear relationship at concentration of 0. 058 7 – 150. 349 5 μg·mL-1(r = 0. 999 7, n = 7). The average recovery rate was 98. 72% , 98. 40% and 98. 60% , respectively; RSD was 1. 84% , 0. 50% and 1. 82% , respectively. The content limitation of (R,S)-epigoitrin was unreasonable according to the transfer rate and the statistical data of the results. Conclusion The method is easy and simple to operate, accurate and stable in results, and highly specific, thus it is applicable for the quality control of Banlangen granules. The content limitation should be determined on the basis of pharmacokinetic and pharmacodynamic data.
