Association between IL-1RN-2018 gene polymorphism and IL-1Ra/IL-1βwith non-small cell lung cancer
10.3969/j.issn.1671-8348.2015.14.020
- VernacularTitle:IL-1RN-2018多态性及IL-1Ra／IL-1β比值与非小细胞肺癌的关系
- Author:
Junrui LI
- Publication Type:Journal Article
- Keywords:
interleukin 1 receptor antagonist;
interleukin 1 bata;
polymorphism;
carcinoma,non-small cell lung
- From:
Chongqing Medicine
2015;(14):1924-1927
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between interleukin receptor antagonist (IL‐1Ra) encoded genes(IL‐1RN‐2018) polymorphisms and serum IL‐1Ra/IL‐1β with non‐small cell lung cancer (NSCLC ) .Methods Totally 85 cases of NSCLC were selected as the NSCLC group and 80 cases of healthy physical examination were selected as the control group from January 2012 to January 2014 .The IL‐1RN‐2018 T/C gene polymorphisms of the two groups were determined with the fluorescence quantitative PCR technique based on high resolution melting ,10% randomly selected samples were sequenced to prove the accura‐cy .The levels of IL‐1Ra and IL‐1βof two groups were determined with ELISA .Results (1)The onset risk of NSCLC in TC and TC+CC genotypes on IL‐1RN‐2018 site in the NSCLC group were increased by 2 .646 times and 2 .315 times respectively compared with TT genotype ,the difference had statistical significance(P<0 .05) .(2)No statistically significant difference of IL‐1RN‐2018 T/C were found between patients with squamous cell carcinoma and adenocarcinomas(P>0 .05) .(3)The serum IL‐1Ra and IL‐1βlev‐els in the NSCLC group were significantly higher than those in the control group ,but IL‐1Ra/IL‐1βin the NSCLC group was signif‐icantly lower (P<0 .05) .(4)The serum IL‐1Ra levels in the NSCLC group had no statistically significant difference among geno‐types (P>0 .05) .Conclusion The C allele in IL‐1RN‐2018 site may increase the onset risk of NSCLC ;the reduction of serum IL‐1Ra/IL‐1βmay presage the risk of NSCLC .
