Study on the effect of small molecule inhibitors LDYS-14007 on JAK1-STAT3 signaling pathway
- VernacularTitle:小分子抑制剂LDYS-14007对JAK1-STAT3信号通路的影响研究
- Author:
Qianqian JI
;
Shangjing GUO
- Publication Type:Journal Article
- Keywords:
LDYS-14007;
JAK1-STAT3 signaling pathways;
rheumatoid arthritis
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(6):10-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of LDYS-14007 on JAK1-STAT3 signaling pathways.Methods MDA-MB-231 cells were treated with 10μmol,1 nmol LDYS-14007, and 10 μmol Tofacitinib,respectively.Western blot assay was used to determine the expression of JAK1,Phospho-JAK1, STAT3 and Phospho-STAT3.Results The absorbance value was linearly related to the concentration of protein C, The linear equation is A=0.0075C+0.0029, r=0.9976, The linear range of 1.08-5.08 mg/mL, With the increased concentration of LDYS-14007, the amount of Phospho-JAK1, Phospho-STAT3 were all gradually decreased.Conclusion LDYS-14007 leads to the levels of Phospho-JAK1 and Phospho-STAT3 decrease, which inhibits JAK1-STAT3 signaling pathway.LDYS-14007 may play an important role in the treatment of rheumatoid arthritis.
